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Oct 10, 2024
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BCH 281L - Biochemistry Lab Credit(s): 2
Prerequisite(s): a grade of “C” or better in CHMY 221 . Corequisite(s): BCH 280 . This laboratory course is designed to be taken concurrently with BCH 280 and is a project-based course that models biochemistry research. Course involves the following: 1. Purification of enzyme from natural sources utilizing high-speed centrifugation, IEX, and affinity chromatography. 2. Characterization of enzyme by gel electrophoresis, Bradford assay, and specific substrate assay. 3. Analysis of enzyme function by kinetic study. (Spring Semester)
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