BCH 281L - Biochemistry Lab

Credit(s): 2

Prerequisite(s): a grade of “C” or better in CHMY 221.
Corequisite(s): BCH 280.
This laboratory course is designed to be taken concurrently with BCH 280 and is a project-based course that models biochemistry research. Course involves the following: 1. Purification of enzyme from natural sources utilizing high-speed centrifugation, IEX, and affinity chromatography. 2. Characterization of enzyme by gel electrophoresis, Bradford assay, and specific substrate assay. 3. Analysis of enzyme function by kinetic study. (Spring Semester)