BCH 281L - Biochemistry Lab

Credit(s): 2

Prerequisite(s): a grade of “C” or better in CHMY 221.
Corequisite(s): BCH 280.
This laboratory course is designed to be taken concurrently with BCH 280 and is a project-based course that models biochemistry research. Course involves the following: 1. Purification of enzyme from natural sources utilizing high-speed centrifugation, IEX, and affinity chromatography. 2. Characterization of enzyme by gel electrophoresis, Bradford assay, and specific substrate assay. 3. Analysis of enzyme function by kinetic study. (Spring Semester)

Course Learning Outcomes

Perform typical laboratory techniques required for conducting biochemistry research.
Engage scientific method to analyze biochemical laboratory data and perform the necessary computations and interpret results.
Produce biochemical laboratory reports using writing as a tool to communicate observations and formulate results and appropriate scientific discussion.